ECL Western Blotting Ultimate Sensitivity Substrate (Femto)
ECL Western Blotting Ultimate Sensitivity Substrate is a non-radioactive chemiluminescent system for detecting horseradish peroxidase (HRP) on immunoblots. Read more
Transported at room temperature, stored at 4°C away from light, shelf life is 12 months.
This product contains an enhanced luminol substrate and a stable peroxide system for detecting femtogram amounts of antigen and is suitable for chemiluminescent detection in conventional Western Blot.
KEY FEATURES
Stability 24-hour working solution stability; 1-year kit stability at room temperature.
High Cost Performance Compared with other chemiluminescent substrates of the same grade, the cost performance is quite high.
High Sensitivity Quickly detect proteins as low as femtogram amounts. Its performance is significantly better than other high-end brand products of the same level.
Ultra-high sensitivity at real femtogram level
The 1 mg/mL actin-HRP antibody was serially diluted to obtain concentrations of 1000, 500, 250, 125, and 62.5 fg/μL. 1μL of each dilution was spotted onto an NC membrane. After spotting, the membrane was developed with YamayBio VQ4726 for 5 minutes.
YamayBio VQ4726 offers higher femtogram-level sensitivity than Thermo 37071.
Lysate: A431 whole cell lysates MW: GAPDH-36kD
Primary antibody: Anti-GAPDH, dilution at 1:5000 Secondary antibody: Goat anti-Mouse IgG(H+L),HRP conjugated at 1:5000 dilution Exposure duration: 10 seconds.
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Specifications
| Product Type | Substrate |
| Detection Method | Chemiluminescence |
| Form | Liquid |
| Application | Western Blot |
| Quantity | 100 mL / 500 mL |
| Sensitivity | Femtogram |
| Membrane Compatibility | Nitrocellulose, PVDF |
