RIPA Lysis Buffer (Strong)
Mainly used to extract soluble proteins from animal tissues and cells. Read more
Transported at room temperature and stored at 4℃. The shelf life is 12 months.
RIPA Lysis Buffer is primarily used to extract soluble proteins from animal cells and tissues and is compatible with diverse applications, including Western Blot, Immunoprecipitation, and Enzyme-Linked Immunosorbent Assay (ELISA). The resulting protein samples are lysed in RIPA buffer, which can then be quantified using the BCA Protein Quantification Kit (Cat.No. BH5484).
KEY FEATURES
Versatile: Enables extraction of proteins from animal cells and tissues, suitable for cytoplasmic, membrane, and nuclear proteins.
Compatible: Supports diverse applications, including Western Blot (WB), Immunoprecipitation (IP), and Enzyme-Linked Immunosorbent Assay (ELISA).
Economical: More cost-effective than other commercial lysis buffers.
Related Products
Protein Sample Preparation
Ready-to-use Bradford Protein Assay Kit (Detergent Compatible)
Product Code: BH5485
Protein Sample Preparation
Ready-to-use BCA Protein Assay Kit
Product Code: BH5484
Protein Sample Preparation
Protease and Phosphatase Inhibitor Cocktail (100×, EDTA-free)
Product Code: BH5483
Protein Sample Preparation
Protease Inhibitor Cocktail, EDTA-free (100×)
Product Code: BH5481
Protein Sample Preparation
Phosphatase Inhibitor Cocktail (100×)
Product Code: BH5482
Protein Sample Preparation
Animal Membrane and Cytosol Protein Extraction Kit
Product Code: BF7475
Protein Sample Preparation
Animal Nuclear and Cytoplasmic Extraction Kit
Product Code: BF7476
Protein Sample Preparation
Lysis Buffer for IP/Co-IP
Product Code: BF7474
Certificate of Analysis
Safety Data Sheet
Show more
Product Manual
Show more
FAQs
Q1 : What samples are suitable for use with RIPA lysis buffer?
A : Used for lysis of mammalian cells and tissues. Not suitable for protein extraction from plants, bacteria, or fungi.
Q2 : Can BCA protein assay kits be used after lysis?
A : Yes
Q3 : Does Lysis Buffer (Cat. No. BF7471 BF7474 )contain protease or phosphatase inhibitors?
A : No
Q4 : What is the clear gelatinous substance present in the lysate after lysis?
A : This is very common.You can shear the DNA by low‑temperature sonication, or adding nuclease
Q5 : Can inhibitors be added to RIPA lysis buffer and stored at 4°C continuously?
A : Not recommended, as protease inhibitors will lose activity quickly。Add fresh before use
Specifications
| Product Type | Buffer |
| Form | Liquid |
| Quantity | 100 mL |
