Nitrocellulose Membranes
Achieve high sensitivity, low background, and superior stripping durability for reliable and cost-effective protein analysis across chemiluminescent and fluorescent applications. Read more
Consumables compatible with Bio-Rad Mini System.
YamayBio PVDF and Nitrocellulose Western Blotting Membranes: reliable solutions for every application. Both deliver low background for clear, consistent results.
Nitrocellulose provides high protein affinity, good sensitivity, and resolution for routine protein analysis.
KEY FEATURES
Unmatched Sensitivity for Rare Targets
Built to Withstand Repeated Stripping
High-Resolution, Ultra-Low Autofluorescence Background
High sensitivity detection across protein loading range
Mouse muscle lysate was separated on a precast gel (AN9325) and transferred using Ice-free Bath Rapid Transfer Buffer (MS8135) at 400 mA for 45 min. Membranes were blocked with Protein-free Blocking Buffer (BM3152) for 15 min, probed with HRP-conjugated antibody (1:25,000, 1 h, RT), and detected by chemiluminescence. Reliable detection down to 0.039 µg protein loading.
Excellent membrane re-probing performance
Mouse muscle lysate, precast gel (AN9325), transferred with Ice-free Bath Rapid Transfer Buffer (MS8135) at 400 mA for 45 min. Blocked with Protein-free Blocking Buffer (BM3152) for 15 min. Probed with GAPDH-HRP (1:25,000, 1 h, RT). Chemiluminescent detection. After initial imaging, membranes were stripped with PS107 buffer and re-probed for four cycles (stripping times: 10, 20, 30, 60 min; total 2 h). Signal integrity maintained through all cycles.
Significantly lower autofluorescence background
HeLa cell lysates were separated on a Bis-Tris 4–20% precast gel and transferred using ice-free transfer buffer. Membranes tested included YamayBio PVDF membrane (0.45 µm) and two MilliporeSigma low-fluorescence PVDF membranes (0.45 µm). After blocking for 15 min, membranes were incubated with primary antibodies against HSP60 or GAPDH, followed by incubation with DyLight 550–labeled secondary antibody or DyLight 488–labeled secondary antibody, respectively.Related Products
Consumables
Nitrocellulose Membranes
Product Code: MS8132
Consumables
PVDF Membranes
Product Code: MS8132
Solutions and Granules
PBS Buffer Instant Granules
Product Code: MS8138
Solutions and Granules
Transfer Buffer Instant Granules
Product Code: MS8134
Protein Electrophoresis
Protein Sample Loading Buffer (Denaturing, non-Reducing, 5×)
Product Code: GB4354
Solutions and Granules
TBS/Tween Buffer Instant Granules
Product Code: MS8133
Protein Electrophoresis
Stain-Free Gradient-like PAGE Gel Kit
Product Code: RF1541
Protein Electrophoresis
Fast-dissolving Dual-color Protein Loading Buffer (Denaturing, Reducing, 5×)
Product Code: GB4353
Solutions and Granules
Protein-free Blocking Buffer (1×)
Product Code: BM3152
Protein Electrophoresis
Stain-Free Bis-Tris Precast Gels
Product Code: AN9321
Solutions and Granules
Ice-free Bath Rapid Transfer Buffer Instant Granules
Product Code: MS8135
Specifications
| Material type | Nitrocellulose |
| Pore size | 0.22 µm / 0.45 µm |
| Dimensions | 30 cm x 3.0 m / 7 x 8.5 cm / 8.5 x 13.5 cm |
| Format | Roll / Sheet |
