Ready-to-use BCA Protein Assay Kit
The Ready-to-use BCA Protein Assay Kit is a highly accurate, detergent-compatible protein assay for determining protein concentration. Read more
Transport in ice packs, store at 4°C, with a shelf life of 12 months.
The Ready-to-use BCA Protein Assay Kit is a highly accurate, detergent-compatible protein assay for determining protein concentration. The Ready-to-use BCA Protein Assay Kit is based on the theory of BCA (Bicinchoninic Acid) to achieve rapid, stable, and sensitive determination of protein concentration.
The principle is that the peptide chain can form a complex with Cu²⁺ in an alkaline environment, reducing Cu²⁺ to Cu⁺ in the process. The BCA reagents bind Cu⁺ specifically and sensitively to form a stable colored complex, which has a high absorbance at 562 nm. The color intensity is proportional to the protein concentration, based on absorbance.
The BCA reagents provide precise protein concentration determination for most sample types, with less interference from factors like metal ions, reducing agents, chelating agents, and detergents commonly encountered in protein research.
KEY FEATURES
Pre-diluted BSA Standards Saving time, reducing chances for pipetting errors.
Good Compatibility Less affected by metal ions, reducing agents, chelating agents, and detergents.
Wide Assay Range A linear working range of 20 to 2000 μg/mL for BSA.
High Accuracy Significantly smaller coefficient of variation compared with the Coomassie Blue Staining method.
High Stability
Stored at 37 °C for 45 days, and after 15 times freezing and thawing, BSA protein standards continue to exhibit excellent performance.
Related Products
Protein Sample Preparation
Ready-to-use Bradford Protein Assay Kit (Detergent Compatible)
Product Code: BH5485
Protein Sample Preparation
Protease and Phosphatase Inhibitor Cocktail (100×, EDTA-free)
Product Code: BH5483
Protein Sample Preparation
Protease Inhibitor Cocktail, EDTA-free (100×)
Product Code: BH5481
Protein Sample Preparation
Phosphatase Inhibitor Cocktail (100×)
Product Code: BH5482
Protein Sample Preparation
Animal Membrane and Cytosol Protein Extraction Kit
Product Code: BF7475
Protein Sample Preparation
Lysis Buffer for IP/Co-IP
Product Code: BF7474
Protein Sample Preparation
RIPA Lysis Buffer (Strong)
Product Code: BF7471
Certificate of Analysis
Batch number
Certificate type
Date
Catalog Number(s)
Controls
Safety Data Sheet
Product Manual
Specifications
| Assay | BCA Assay |
| Product Type | Protein Quantitation Assay |
| Form | Liquid |
| Sufficient For | 500 Assays / 2500 Assays |
| Reagent Type | BCA Reagent A / BCA Reagent B / Protein Standards 1 - 8 |
