Animal Nuclear and Cytoplasmic Extraction Kit
This kit enables the convenient and efficient separation of nuclear proteins and cytoplasmic proteins from animal cells or tissues. It gradually lyses cells, separates the intact nuclei from the cytoplasm, and then extracts nuclear proteins from the isolated nuclei. Read more
Transported in ice packs and stored at 4°C. The shelf life is 12 months.
This kit utilizes optimized detergent formulations to enable rapid and efficient isolation of nuclear and cytoplasmic proteins from animal cells. The nuclear fraction includes nuclear membrane proteins as well as soluble nuclear proteins, such as histones and transcription factors. The protocol reduces nuclear protein extraction time to approximately 10 minutes while delivering significantly higher yields compared to conventional extraction kits.
The system provides efficient nuclear-cytoplasmic separation, making it particularly suitable for Western Blot (WB) analysis.
KEY FEATURES
High Purity Cross-contamination between nuclear and cytoplasmic fractions is maintained below 5%.
Convenient Workflow No gradient centrifugation required
Rapid Protocol (High-efficiency for WB) Total extraction completed in approximately 1 hour
Unparalleled Yield (High-efficiency for WB) This kit is an efficient Western Blot reagent kit with an optimized formulation for nuclear protein extraction, yielding more protein than conventional protein extraction kits, making it ideal for Western Blot applications. Achieve up to 50% greater output.
High Yield (Multi-application Type) Effectively extracts soluble nuclear proteins (excluding nuclear membrane proteins).
High Compatibility (Multi-application Type) Suitable for experiments such as WB, IP, EMSA, Footprinting, reporter gene assays, and enzyme activity assays.
Unparalleled Yield
Fig 1. Protein concentration was determined using the Ready-to-use BCA Protein Assay Kit (CAT. No.BH5484).Values represent the average of two independent isolations.The kit yields approximately 200-300 μg of cytoplasmic protein and 50-150 μg of nuclear protein at 1 mg/mL.
Fig 2. Western Blot analysis of nuclear proteins fractions extracted using YamayBio BF7476 and Thermo 78833. Equal numbers of Expi293F cells (2 × 10^6) were processed according to each manufacturer's instructions.
Compared to the Thermo kit, YamayBio BF7476 demonstrated higher recovery of nuclear proteins including Histone H3, Histone H2A.X, and c-Fos, while maintaining comparable cytoplasmic protein yields.
High Purity
Fig 3. Western Blot analysis of cytoplasmic proteins fractions extracted using YamayBio BF7476 and Thermo 78833.Equal numbers of Expi293F cells (2 × 10^6) were processed according to each manufacturer's protocol. Cytosolic (C) and nuclear (N) fractions were analyzed using specific marker antibodies to evaluate extraction efficiency and cross-contamination. GAPDH was used as a cytosolic marker, and HSP60 as a mitochonndrial marker to assess fraction purity.
Ensures minimal cross-contamination between nuclear and cytoplasmic fractions, with contamination levels typically below 5%, resulting in high-quality nuclear protein extracts suitable for downstream applications.
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Certificate of Analysis
Safety Data Sheet
Product Manual
FAQs
Q1 : What are the functions of each component in BF7476 and BF7477?
A : The Reagent A induces swelling of the cell causing stress on the cellular membrane. The Reagent B reagent gently lyses the cell membrane allowing cytoplasmic proteins to be collected, while leaving the nucleus intact. The Reagent C is then used to extract nuclear proteins from the pellet.
Q2 : What is the difference between BF7476 and BF7477?
A : BF7476 contains detergent. The extracted nuclear proteins include nuclear membrane proteins, as well as histones and intranuclear transcription factors. It suitable for Western blot analysis.
BF7477 is detergent-free. It efficiently extracts soluble intranuclear proteins while excluding nuclear membrane proteins. After desalting or dilution, the isolated proteins are compatible with WB, IP, EMSA, Footprinting assays, reporter gene detection, and enzyme activity measurements.
Q3 : How can I minimize cross-contamination of proteins between the nuclear and cytoplasmic fractions when using our kits？
A : Careful pipetting and performing the incubation and centrifugation as indicated in the instructions will minimize cross-contamination. Performing an additional wash of the nuclear pellet with PBS before adding the Reagent C will reduce carryover of the cytoplasmic proteins to the nuclear protein fraction.
Specifications
| Product Type | Nuclear and Cytoplasmic Extraction Kit |
| Form | Liquid |
| Sufficient For | 50 Samples / 250 Samples |
| Shelf Life | 12 Months. |
| Storage Requirements | 4°C |
