UltraPure Mitochondria and Mitochondrial Protein Extraction Kit
This kit isolates intact mitochondria within 60 minutes, using a two-step density-gradient centrifugation method. The extracted mitochondria retain 11 intact double-membrane structures and high biological activity. They are suitable for downstream applications such as apoptosis analysis, signal transduction, metabolic studies, and mitochondrial proteomics. Read more
Transported in ice packs and stored at 4°C. The shelf life is 12 months.
This kit isolates intact mitochondria within 60 minutes using a two-step density gradient centrifugation method. The extracted mitochondria possess complete double-membrane structures and retain high biological activity. They are suitable for downstream applications such as apoptosis analysis, signal transduction, metabolic studies, and mitochondrial proteomics.
KEY FEATURES
High Purity The two-step density gradient separation ensures high-puritymitochondrial isolation with minimal contamination from other cellular components
High Activity Isolated mitochondria retain intact structure and biological activity, making them suitable for functional and enzymatic studies.
Wide Application
Each mitochondria are suitable for:
· Mitochondrial function and enzyme activity studies
· Mitochondrial DNA (mtDNA) extraction
· Mitochondrial protein analysis (SDS-PAGE, Western Blot (WB), Immunoprecipitation (IP), and proteomics
research)
High Purity
A total of 100 mg of mouse liver tissue was processed following the kit protocol to generate the following fractions: liver homogenate, nuclear or unlysed cell pellet, cytosolic supernatant, crude mitochondria, and purified mitochondria.Each fraction was diluted 1:100 in PBS and analyzed by flow cytometry.
As shown in Fig 1, the purified mitochondrial fraction exhibited a distinct and uniform cluster distribution, indicating high mitochondrial purity.
Mitochondria extracted from 100 mg of mouse liver tissue using BF7478 were stained with Janus Green solution.
Microscopic examination revealed blue-green stained, rod-shaped or dumbbell-shaped mitochondria, with occasional aggregation due to the high mitochondrial density in liver tissue.
High Activity
Isolated mitochondria were stained with MitoTracker Red, a membrane potential-dependent fluorescent dye, and observed under a fluorescence microscope.As shown in Figure 3, the mitochondrial suspension emitted red fluorescence, indicating preserved membrane and high mitochondrial activity.
Wide Application
The isolated mitochondria are suitable for mitochondrial function studies, mitochondrial protein analysis, and mtDNA extraction.As shown in Figure 4, COX IV and Hsp60 bands were significantly wnriched in the mitochondrial fraction compared to total protein samples, confirming effective mitochondrial isolation using BF7478. No Lamin A/C or Syntaxin 16 bands were detected in the mitochondrial fraction, indicating minimal nuclear or Golgi contamination.
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Product Manual
Specifications
| Product Type | UltraPure Mitochondria and Mitochondrial Protein Extraction Kit |
| Form | Liquid |
| Sufficient For | 30 Samples |
| Shelf Life | 12 Months |
| Storage Requirements | 4°C |
