UltraPure Mitochondria and Mitochondrial Protein Extraction Kit
This kit isolates intact mitochondria within 60 minutes, using a two-step density-gradient centrifugation method. The extracted mitochondria retain 11 intact double-membrane structures and high biological activity. They are suitable for downstream applications such as apoptosis analysis, signal transduction, metabolic studies, and mitochondrial proteomics. Read more
Transported in ice packs and stored at 4°C. The shelf life is 12 months.
This kit isolates intact mitochondria within 60 minutes using a two-step density gradient centrifugation method. The extracted mitochondria possess complete double-membrane structures and retain high biological activity. They are suitable for downstream applications such as apoptosis analysis, signal transduction, metabolic studies, and mitochondrial proteomics.
KEY FEATURES
High Purity The protocol uses a two-step density gradient centrifugation method, ensuring a higher purity of the isolated mitochondria.
High Activity The isolated mitochondria retain their intact structure and exhibit high biological activity.
Wide Application
The mitochondria extracted using this product can be used for:
· Mitochondrial function or enzyme activity studies
· Mitochondrial DNA (mtDNA) extraction
· Mitochondrial protein analysis, such as SDS-PAGE, Western Blot (WB), Immunoprecipitation (IP), and proteomics research
High Purity
100 mg of mouse liver tissue was processed according to the protocol to obtain the following fractions: liver homogenate, pellet (containing nuclei, unlysed cells, etc.), supernatant (cytoplasmic components), crude mitochondria, and purified mitochondria. Each fraction was diluted 1:100 in PBS and analyzed by flow cytometry.As shown in Fig 1, the purified mitochondrial fraction displayed a distinct, uniformly distributed cluster, indicating a high degree of mitochondrial purity.
Mitochondria extracted from 100mg mouse liver tissue using BF7478 were stained with Janus Green solution.
Microscopic examination showed: Blue-green stained mitochondria appearing as rod-shaped or dumbbell-shaped structures, with frequent aggregation due to high mitochondrial density in liver tissue.
High Activity
The isolated mitochondria were treated with MitoTracker Red, a membrane potential-dependent mitochondrial dye, and observed under a fluorescence microscope.As shown in Figure 3, the stained mitochondrial suspension emitted red fluorescence, indicating that the mitochondrial membrane potential was well preserved and the extracted mitochondria remained highly active.
Wide Application
The extracted mitochondria can be used for mitochondrial function studies, mitochondrial protein analysis, and mtDNA extraction.As shown in Figure 4, COX IV and Hsp60 bands were much stronger in the mitochondrial fraction than in the total protein fraction, showing effective enrichment by BF7478.
No Lamin A/C or Syntaxin 16 bands were detected, indicating minimal contamination from nuclei or Golgi.
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Specifications
| Product Type | UltraPure Mitochondria and Mitochondrial Protein Extraction Kit |
| Form | Liquid |
| Sufficient For | 30 Samples |
| Shelf Life | 12 Months |
| Storage Requirements | 4°C |
