Western Blot Rapid Stripping Buffer (mild and general type)
Essential tools for removing primary and secondary antibodies from membranes. Read more
Transported at room temperature and stored at 4°C. The shelf life is 12 months.
YamayBio Western Blot Rapid Stripping buffers are essential tools for removing primary and secondary antibodies from membranes, enabling reprobing, saving time and costs when sample quantities are limited . YamayBio Western Blot Rapid Stripping Buffer is specifically formulated to gently and efficiently removes bound antibodies from PVDF and NC membranes, minimizing damage to the membrane. Its rapid action and odorless, environmentally friendly formulation ensure a safe and effective process.
KEY FEATURES
Efficient Strippin Superior performance compared to conventional stripping buffer
Mild Yet Effective
Removes bound antibodies without stripping
Superior performance compared to conventional
stripping buffer target proteins
The mild formulation of YamayBio Rapid Stripping buffer minimizes damage to antigens and membranes.
Fig 1. Hela cells lysate (2.5μg, 1.25μg, 0.62μg, 0.31μg of total protein) were probed with GAPDH. Following the initial detection,each blot was divided into two strips, one strip was treated with YamayBio BM3154, and the other with Thermo Scientific Restore Western Blot Stripping buffer(Part No.21059), for extended stripping(6 hours). After stripping, membrane were washed in 1X PBS Tween-20, and incubated with the substrate, and imaged.Membrane were then reblocked and reprobed for GAPDH, the Western blot procedure repeated as described above.
Results demonstrate that BM3154 perserves antigen integrity even after prolonged stripping.
The Rapid Stripping Buffer is fully compatible with both PVDF and Nitrocellulose (NC) membranes.
Fig 2. A431 cell lysates (2.5μg,1.25μg,0.62μg,0.31μg of total protein) were separated by SDS-PAGE and transferred onto PVDF or nitrocellulose (NC) membranes. Membranes were blocked by Protein-Free Blocking Buffer (Part No.BM3152) and probed with an anti-GAPDH antibody. Following initial detection, each blot was divided into two strips. One strip was treated with YamayBio Rapid Stripping Buffer (BM3154), while the other was treated with Thermo Scientific RestoreTM Western Blot Stripping Buffer (Part No.21059) for 15 minutes at room temperature, according to the manufacturer's instructions. After stripping procedure, membranes were washed in 1X PBS Tween-20, with incubated with substrate, and imaged. Membrane were then reblocked and reprobed anti-HSP60 antibody.
Results confirmed efficient antibody removal with minimal antigen loss on both PVDF and nitrocellulose membranes.
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Specifications
| Product Type | Western Blot Stripping Buffer |
| Form | Liquid |
| Application | Western Blot |
| Membrane Compatibility | Nitrocellulose, PVDF |
| Quantity | 100 mL / 500 mL |
