Antibody Diluent
Antibody Dilution is an efficient diluent containing various antibody protective agents. Read more
Transported at room temperature and stored at 4°C. The shelf life is 12 months.
Antibody Dilution is an efficient diluent containing various antibody protective agents. The dilution buffer has the properties of enhancing antibody activity and stability, reducing non-specific binding, and improving the specificity and sensitivity of experiments.
KEY FEATURES
Suitable for Western blot, Immunofluorescence and Immunohistochemistry.
Enhanced Antibody Stability And Efficiency
Antibodies diluted with this antibody diluent can be stored at 4°C for
up to 6 months without the need for repeated freeze-thaw cycles.
Highly Versatile No cross-reactivity with antibodies or target proteins, higher signal-noise ratio compared to non-fat milk and BSA. Suitable for the detection for phosphoproteins.
Quick Start Protocol
1. Calculate the required volumes of antibody and Universal Antibody Dilution Buffer based on the appropriate dilution ratio. The final volume should be sufficient to completely cover the membrane in the incubation box. Typically, 5–10 mL of dilution buffer is recommended.
2.Transfer the calculated volume of Universal Antibody Dilution Buffer into a 15 mL or 50 mL centrifuge tube, depending on the total volume required. Then, add the corresponding volume of antibody to the buffer.
3.Close the cap tightly and gently invert, tip, and swirl the tube several times to mix.
4.Carefully pour the prepared antibody solution into the incubation box, ensuring the membrane is fully covered.
Fig1. Accelerated stability testing of the primary antibody and HRP-conjugated secondary antibody after dilution with antibody diluent (YamayBio Cat.NO.: BM3153). a. Rabbit Anti-GAPDH antibody and b. Goat anti-Rabbit IgG (H + L), HRP-conjugated antibodies were diluted 1:5000 with antibody diluent. The diluted antibodies were then aliquoted into two tubes and stored at 4°C and 37°C for 14 days, respectively. Subsequently, Western Blot analysis was performed. As shown in the figures, the results indicated that the WB bands obtained with the GAPDH antibody and the HRP-conjugated secondary antibody stored at 37°C for 14 days were comparable to those stored at 4°C for 14 days.
Fig2. Immunofluorescence staining for PSD95 was performed on mouse brain tissue sections using an anti-PSD95 primary antibody diluted 1:100 in antibody diluent (YamayBio Cat. No.:BM3153). A goat anti-rabbit secondary antibody (green fluorescence) was diluted
1:1000 in the same diluent.
Fig3. Formalin-fixed, paraffin-embedded mouse uterine tissue sections were undergo immunohistochemical staining for ER alpha. Anti-ER alpha antibody was diluted in antibody diluent (YamayBio Cat. No.:BM3153) prior to incubation with the tissue sections.
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Certificate of Analysis
Safety Data Sheet
Product Manual
Specifications
| Product Type | Buffer |
| Form | Liquid |
| Quantity | 100 mL / 500 mL |
