Protein-free Blocking Buffer (1×)
Protein-Free Rapid Blocking Buffer is a non-protein-based compound that provides effective blocking for membrane- and plate-based protein detection methods, resulting in extremely low background. Read more
Transported at room temperature and stored at 4°C. The shelf life is 12 months.
Protein-Free Rapid Blocking Buffer is a non-protein-based compound that provides effective blocking for membrane- and plate-based protein detection methods, resulting in extremely low background.
Traditional blocking buffers contain proteins that can cross-react in certain immunodetection systems, resulting in high background and reduced signal.Protein-Free Rapid Blocking Buffer contains no endogenous phosphorylated proteins or biotin, resulting in no interference in these detection systems.The blocking buffer does not produce non-specific binding to the protein, blocking only the membrane without shielding the antibody recognition sites on the sample proteins. This greatly improves antibody detection sensitivity.
KEY FEATURES
Rapid and Efficient Complete blocking in 5-10 minutes.
Protein Free No cross-reactivity with antibodies or target proteins, higher signal-noise ratio compared to non-fat milk and BSA. Suitable for the detection for phosphoproteins.
Applicable to phosphorylated proteins
Higher signal-noise ratio compared to non-fat milk and BSA
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Specifications
| Product Type | Blocking Buffer |
| Form | Liquid |
| Application | Western Blot |
| Concentration | 1× |
| Quantity | 500 mL |
