Animal Nuclear and Cytoplasmic Extraction Kit
This kit enables the convenient and efficient separation of nuclear proteins and cytoplasmic proteins from animal cells or tissues. It gradually lyses cells, separates the intact nuclei from the cytoplasm, and then extracts nuclear proteins from the isolated nuclei. Read more
Transported in ice packs and stored at 4°C. The shelf life is 12 months.
This kit utilizes optimized detergent formulations to enable rapid and efficient isolation of nuclear and cytoplasmic proteins from animal cells. The nuclear fraction includes nuclear membrane proteins as well as soluble nuclear proteins, such as histones and transcription factors. The protocol reduces nuclear protein extraction time to approximately 10 minutes while delivering significantly higher yields compared to conventional extraction kits.
The system provides efficient nuclear-cytoplasmic separation, making it particularly suitable for Western Blot (WB) analysis.
KEY FEATURES
High Purity Cross-contamination between nuclear and cytoplasmic fractions is maintained below 5%.
Convenient Workflow No gradient centrifugation required
Rapid Protocol (High-efficiency for WB) Total extraction completed in approximately 1 hour
Unparalleled Yield (High-efficiency for WB) This kit is an efficient Western Blot reagent kit with an optimized formulation for nuclear protein extraction, yielding more protein than conventional protein extraction kits, making it ideal for Western Blot applications. Achieve up to 50% greater output.
High Yield (Multi-application Type) Effectively extracts soluble nuclear proteins (excluding nuclear membrane proteins).
High Compatibility (Multi-application Type) Suitable for experiments such as WB, IP, EMSA, Footprinting, reporter gene assays, and enzyme activity assays.
Unparalleled Yield
Fig 1. Protein concentration was determined using the Ready-to-use BCA Protein Assay Kit (CAT. No.BH5484).Values represent the average of two independent isolations.The kit yields approximately 200-300 μg of cytoplasmic protein and 50-150 μg of nuclear protein at 1 mg/mL.
Fig 2. Western Blot analysis of nuclear proteins fractions extracted using YamayBio BF7476 and Thermo 78833. Equal numbers of Expi293F cells (2 × 10^6) were processed according to each manufacturer's instructions.
Compared to the Thermo kit, YamayBio BF7476 demonstrated higher recovery of nuclear proteins including Histone H3, Histone H2A.X, and c-Fos, while maintaining comparable cytoplasmic protein yields.
High Purity
Fig 3. Western Blot analysis of cytoplasmic proteins fractions extracted using YamayBio BF7476 and Thermo 78833.Equal numbers of Expi293F cells (2 × 10^6) were processed according to each manufacturer's protocol. Cytosolic (C) and nuclear (N) fractions were analyzed using specific marker antibodies to evaluate extraction efficiency and cross-contamination. GAPDH was used as a cytosolic marker, and HSP60 as a mitochonndrial marker to assess fraction purity.
Ensures minimal cross-contamination between nuclear and cytoplasmic fractions, with contamination levels typically below 5%, resulting in high-quality nuclear protein extracts suitable for downstream applications.
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Certificate of Analysis
Safety Data Sheet
Product Manual
Specifications
| Product Type | Nuclear and Cytoplasmic Extraction Kit |
| Form | Liquid |
| Sufficient For | 50 Samples / 250 Samples |
| Shelf Life | 12 Months. |
| Storage Requirements | 4°C |
